Two Types of Vesicles from the Erythrocyte-Ghost Membrane Differing in Surface Charge.

Vesicles of erythrocyte ghosts were produced by treatment with Primaquine or hexokinase. The resulting vesicles could be separated from each other and characterized by preparative free-flow electrophoresis. One population consisted of outside-out vesicles carrying the same sialic acid distribution as the original ghosts. The other represented homogeneous inside-out particles with a different electrophorctic mobility carrying most of their sialic acid at their inner side. The two kinds of vesicles could not be separated by density gradient centrifugation. [ABSTRACT FROM AUTHOR]