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BIOINFORMATIC ANALYSIS OF DOSE- AND TIME-DEPENDENT miRNome RESPONSES.

Authors :
Babini, G
Tanno, B
Stefano, I De
Giardullo, P
Leonardi, S
Pasquali, E
Baiocco, G
Ottolenghi, A
Mancuso, M
Source :
Radiation Protection Dosimetry; May2019, Vol. 183 Issue 1/2, p151-155, 5p
Publication Year :
2019

Abstract

The advent of new 'omics' techniques determined a massive boost in the measurement of the whole spectra of molecules within cells, favoring promising new radiobiological studies at low doses. The main aim of this work was to assess the radiation-induced perturbations of miRNA profiles and their temporal dynamics. Human Umbilical Vein Endothelial Cells were irradiated with low doses of γ-rays. At different time points post-irradiation, cells were harvested and miRNAs isolated. A full mapping of the miRNA sequences via Next-Generation-Sequencing analysis was performed followed by bioinformatic analyses. Pathway enrichment analyses on the differentially expressed miRNAs focused both on the averaged effects of different doses over the 24-h experiment and on the altered temporal dynamics of the miRNA profiles. These complementary analyses provided a picture of the dose- and time-dependent miRNAs responses, allowing to better explore the candidate biomarkers linked to radiation exposures and their corresponding pathways and functions. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
01448420
Volume :
183
Issue :
1/2
Database :
Complementary Index
Journal :
Radiation Protection Dosimetry
Publication Type :
Academic Journal
Accession number :
136525495
Full Text :
https://doi.org/10.1093/rpd/ncy215