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Macrophage inflammatory protein 1 alpha expression by synovial fluid neutrophils in rheumatoid arthritis.
- Source :
- Annals of the Rheumatic Diseases; May1999, p297-302, 6p
- Publication Year :
- 1999
-
Abstract
- <bold>Objective: </bold>To determine the contribution made by synovial fluid (SF) neutrophils to the augmented expression of macrophage inflammatory protein 1 alpha (MIP-1alpha) in rheumatoid arthritis (RA).<bold>Methods: </bold>Neutrophils were isolated from samples of SF from RA patients and peripheral blood (PB) samples from RA patients and healthy controls. Cell associated MIP-1alpha was visualised immunohistochemically, and cell associated MIP-1alpha as well as MIP-1alpha secreted into the SF was assayed by ELISA. Steady state expression of MIP-1alpha mRNA was assessed by reverse transcription polymerase chain reaction (RT-PCR).<bold>Results: </bold>Freshly isolated SF neutrophils contained significantly higher concentrations of both MIP-1alpha protein and its transcript than PB neutrophils from either RA patients or healthy controls; incubation in the absence or presence of tumour necrosis factor alpha for 24 hours resulted in a significant increase in MIP-1alpha secretion by RA SF neutrophils compared with neutrophils obtained from either normal PB or RA PB; and expression of MIP-1alpha by SF neutrophils was well correlated with both RA disease activity and SF mononuclear cell (MNC) counts.<bold>Conclusion: </bold>Expression and secretion of MIP-1alpha by SF neutrophils may be indicative of local and systemic inflammation in RA. Moreover, this C-C chemokine may contribute to the recruitment of MNCs from the bloodstream into synovial joints and tissues. [ABSTRACT FROM AUTHOR]
- Subjects :
- PROTEIN analysis
RNA analysis
COMPARATIVE studies
CYTOKINES
ENZYME-linked immunosorbent assay
IMMUNOHISTOCHEMISTRY
RESEARCH methodology
MEDICAL cooperation
NEUTROPHILS
NONPARAMETRIC statistics
POLYMERASE chain reaction
PROTEINS
RESEARCH
RHEUMATOID arthritis
SYNOVIAL fluid
EVALUATION research
REVERSE transcriptase polymerase chain reaction
Subjects
Details
- Language :
- English
- ISSN :
- 00034967
- Database :
- Complementary Index
- Journal :
- Annals of the Rheumatic Diseases
- Publication Type :
- Academic Journal
- Accession number :
- 136427992
- Full Text :
- https://doi.org/10.1136/ard.58.5.297