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Extracts of Celastrus Orbiculatus Inhibit Cancer Metastasis by Down-regulating Epithelial-Mesenchymal Transition in Hypoxia-Induced Human Hepatocellular Carcinoma Cells.
- Source :
- Chinese Journal of Integrative Medicine; May2019, Vol. 25 Issue 5, p334-341, 8p
- Publication Year :
- 2019
-
Abstract
- Objective: To evaluate the effects of Celastrus Orbiculatus extracts (COE) on metastasis in hypoxia-induced hepatocellular carcinoma cells (HepG2) and to explore the underlying molecular mechanisms. Methods: The effect of COE (160, 200 and 240 µ g/mL) on cell viability, scratch-wound, invasion and migration were studied by 3-4,5-dimethyl-2-thiazolyl-2,5-diphenyl-2-H-tetrazolium bromide (MTT), scratch-wound and transwell assays, respectively. CoCl<subscript>2</subscript> was used to establish a hypoxia model in vitro. Effects of COE on the expressions of E-cadherin, vimentin and N-cadherin were investigated with Western blot and immunofluorescence analysis, respectively. Results: COE inhibited proliferation and metastasis of hypoxia-induced hepatocellular carcinoma cells in a dose-dependent manner (P<0.01). Furthermore, the expression of epithelial-mesenchymal transition (EMT) related markers were also remarkably suppressed in a dose-dependent manner (P<0.01). In addition, the upstream signaling pathways, including the hypoxia-inducible factor 1 α (Hif-1 α) and Twist1 were suppressed by COE. Additionally, the Hif-1 α inhibitor 3-5′-hydroxymethyl-2′-furyl)-1-benzylindazole (YC-1), potently suppressed cell invasion and migration as well as expression of EMT in hypoxia-induced HepG2 cells. Similarly, the combined treatment with COE and YC-1 showed a synergistic effect (P<0.01) compared with the treatment with COE or YC-1 alone in hypoxia-induced HepG2 cells. Conclusions: COE significantly inhibited the tumor metastasis and EMT by suppressing Hif-1 α/Twist1 signaling pathway in hypoxia-induced HepG2 cell. Thus, COE might have potential effect to inhibit the progression of HepG2 in the context of tumor hypoxia. [ABSTRACT FROM AUTHOR]
- Subjects :
- METASTASIS
HYPOXEMIA
BIOCHEMISTRY
CELL physiology
CELLULAR signal transduction
COBALT
CYTOSKELETAL proteins
HUMAN embryology
EPITHELIAL cells
FLUORESCENT antibody technique
GENE expression
GLYCOPROTEINS
HEPATOCELLULAR carcinoma
HETEROCYCLIC compounds
PHENOMENOLOGY
MEDICINAL plants
PROTEINS
WESTERN immunoblotting
PLANT extracts
TREATMENT effectiveness
CELL survival
CELL migration inhibition
IN vitro studies
CHEMICAL inhibitors
PREVENTION
Subjects
Details
- Language :
- English
- ISSN :
- 16720415
- Volume :
- 25
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- Chinese Journal of Integrative Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 136274199
- Full Text :
- https://doi.org/10.1007/s11655-018-2562-9