Back to Search
Start Over
Quantitative Analysis of the Proton and Charge Stoichiometry of Cytochrome <em>c</em> Oxidase from Beef Heart Reconstituted into Phospholipid Vesicles.
- Source :
- European Journal of Biochemistry; 10/15/80, Vol. 111 Issue 2, p299-306, 8p, 3 Diagrams, 2 Graphs
- Publication Year :
- 1980
-
Abstract
- The proton and charge stoichiometry of cytochrome c oxidase reconstituted into phospholipid vesicles has been analysed using a fast responding oxygen electrode and a system for the simultaneous measurement of H<superscript>-</superscript>, K<superscript>+</superscript> or lipophilic cations, and O<subscript>2</subscript>. From initial rate measurements after addition of reductant (ascorbate,′′N,N,N′,N′-tetramethyl-p-phenylenediamine) the following stoichiometries could be extrapolated: K<superscript>+</superscript>/e<superscript>-</superscript>, between 1.85 and 2.05, H<superscript>+</superscript>/e<superscript>-</superscript>, between 0.58 and 0.75. Lipophilic cations can replace valinomycin potassium as the charge compensating system. Net H<superscript>+</superscript> extrusion was observed for up to 11 turnovers of the enzyme (11 O<subscript>2</subscript>/cytochrome aa<subscript>3</subscript>). The variation of the internal pH buffering capacity of the vesicles has an influence on the number of turnovers during which net proton translocation can be observed, but has no influence on the determined stoichiometries. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00142956
- Volume :
- 111
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- European Journal of Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 13618316
- Full Text :
- https://doi.org/10.1111/j.1432-1033.1980.tb04942.x