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Property and Activity of Mycoloyl Esters of Methyl Glucoside and Trehalose.
- Source :
- European Journal of Biochemistry; 2/15/79, Vol. 94 Issue 1, p109-118, 10p
- Publication Year :
- 1979
-
Abstract
- Methyl α-D-glucoside and α,α'-D-trehalose acylated on their primary hydroxyl group by α-alkyl-β-hydroxy acids (mycolic acids) with 18, 32 and more than 80 carbon atoms were studied. These cord factors and analogues when dispersed in water gave hydrated and organized phases which bore resemblances and differences to phospholipid liposomes. Phase transition temperature was determined by fluorescence for the C<subscript>32</subscript> derivatives and by differential scanning calorimetry for the C<subscript>80</subscript> derivatives. A narrow temperature interval between the gel and the fluid states was observed, indicating that this transition was a strongly cooperative phenomenon. The activity on mitochondria of these diacyl trehaloses was studied with succinate as substrate. The fluid suspensions were more active than the rigid ones. There was no influence of the relative configuration of the mycoloyl residue. Acyl chains with 18, 32 and more than 80 carbon atoms had rather similar activities on phosphorylation, but differences appeared when considering effects on respiration. Electron microscopy suggested that cord factor weakened the peripheral part of the inner membrane, but cristae looked unchanged. These observations, together with the results of our previous monolayer studies of these glycolipids, led us to propose a model for interaction of cord factor with mitochondria. [ABSTRACT FROM AUTHOR]
- Subjects :
- ORGANIC compounds
CARBON
CALORIMETRY
OPTICS
FLUIDS
ESTERS
Subjects
Details
- Language :
- English
- ISSN :
- 00142956
- Volume :
- 94
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- European Journal of Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 13606168
- Full Text :
- https://doi.org/10.1111/j.1432-1033.1979.tb12877.x