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Sources of variability in SERS spectra of bacteria: comprehensive analysis of interactions between selected bacteria and plasmonic nanostructures.

Authors :
Witkowska, Evelin
Niciński, Krzysztof
Korsak, Dorota
Szymborski, Tomasz
Kamińska, Agnieszka
Source :
Analytical & Bioanalytical Chemistry; Apr2019, Vol. 411 Issue 10, p2001-2017, 17p
Publication Year :
2019

Abstract

The surface-enhanced Raman spectroscopy (SERS)-based analysis of bacteria suffers from the lack of a standard SERS detection protocol (type of substrates, excitation frequencies, and sampling methodologies) that could be employed throughout laboratories to produce repeatable and valuable spectral information. In this work, we have examined several factors influencing the spectrum and signal enhancement during SERS studies conducted on both Gram-negative and Gram-positive bacterial species: Escherichia coli and Bacillus subtilis, respectively. These factors can be grouped into those which are related to the structure and types of plasmonic systems used during SERS measurements and those that are associated with the culturing conditions, types of culture media, and method of biological sample preparation. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
16182642
Volume :
411
Issue :
10
Database :
Complementary Index
Journal :
Analytical & Bioanalytical Chemistry
Publication Type :
Academic Journal
Accession number :
135841981
Full Text :
https://doi.org/10.1007/s00216-019-01609-4