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Immobilization of pigeonpea (Cajanus cajan) urease on DEAE-cellulose paper strips for urea estimation.
- Source :
- Biotechnology & Applied Biochemistry; Jun2004, Vol. 39 Issue 3, p323-327, 5p
- Publication Year :
- 2004
-
Abstract
- Pigeonpea ( Cajanus cajan ) urease was immobilized on 1 cm×1 cm DEAE-cellulose paper strips. The optimum immobilization (51% activity) was observed at 4 °C, with a protein concentration of 1.0 mg/strip. The apparent optimum pH shifted from 7.3 to 6.8. Immobilized urease showed an optimal stability temperature of 67 °C, compared with 47 °C for the soluble urease. Time-dependent kinetics of the thermal inactivation of the immobilized urease were examined and found to be monophasic as compared with the soluble enzyme, which was biphasic. The Michaelis constant ( K <subscript>m</subscript>) for the DEAE-cellulose-immobilized urease was found to be 4.75 mM, 1.5 times higher than the soluble enzyme. Immobilized strips stored at 4 °C showed an increased half-life ( t <subscript>1/2</subscript>=150 days). There was practically no leaching of the enzyme from the immobilized strips over a period of 2 weeks. These strips were used for estimating the urea content of blood samples; the results obtained matched well with those obtained in a clinical laboratory through an Autoanalyzer® (Zydus Co., Rome, Italy). The easy availability of pigeonpea urease, the ease of its immobilization on DEAE-cellulose strips and the significantly lower cost of urease described in the present study makes it a suitable product for future applications in diagnostics. [ABSTRACT FROM AUTHOR]
- Subjects :
- PIGEON pea
UREASE
CELLULOSE
AMIDASES
PROTEINS
Subjects
Details
- Language :
- English
- ISSN :
- 08854513
- Volume :
- 39
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Biotechnology & Applied Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 13559904