Isolation of thermophilic Anoxybacillus beppuensis JF84 and production of thermostable amylase utilizing agro–dairy wastes.

Extremophiles are the organisms that can be manipulated for various industrial activities. Agro–dairy wastes are being utilized for economical production of enzymes employing microbes. In this regard, thermophilic amylolytic bacterium was isolated from soil in vicinity of hot spring near Chakwal, Pakistan. The isolated bacterium was rod‐shaped, positive for Gram staining, and endospores former. The isolate was able to utilize sugars like, maltose, xylose, fructose, sachorose, sorbitol, mannose, mannitol, arabinose and polysaccharides like gelatin and starch. The isolate grew over a wide temperature range (40–70°C) and pH (4–9). It exhibited enough growth with different nitrogen sources which could produce amylase up to 0.72 U/mL, while hydrolyzing starch. Phylogenetic analysis based on 16S rRNA gene sequence showed that it belongs to Anoxybacillus beppuensis under Accession number KF254912 (JF84). The DNA G+C content were 56.59%. The isolate JF84 showed fourfold purification and percentage yield was estimated to be 94.21% as compared to crude enzyme. The decreasing order of substrate suitability for amylase production was found to be sugarcane bagasse (SCB) > wheat straw (WS) > sugarcane bagasse and whey (SB + W) > whey (W). A. beppuensis JF84 yielded enough thermostable α‐amylase in economical substrates as well as tolerated broad pH and temperature suggesting its wide economical commercial use in amylase‐dependent industries. © 2018 American Institute of Chemical Engineers Environ Prog, 38: 417–423, 2019 [ABSTRACT FROM AUTHOR]