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Analytical-Band Centrifugation of an Active Enzyme-Substrate Complex.
- Source :
- European Journal of Biochemistry; 1971, Vol. 23 Issue 2, p267-275, 9p
- Publication Year :
- 1971
-
Abstract
- The analytical active-enzyme-centrifugation method permits the determination of the hydrodynamic properties of enzymes while they are fully active on their substrates: the centrifugation can be performed at the very dilute concentrations used in kinetic studies (&aysmp; 1 µg/ml): furthermore a purified enzyme preparation is not necessary and in many cases a crude extract is sufficient. This method is beginning to be used in several laboratories even though few of its experimental aspects have been published. This paper reports the details for carrying out, an activeenzyme-centrifugation run, discusses the experimental conditions anti details with artifacts and ways, which are easy, to overcome them. [ABSTRACT FROM AUTHOR]
- Subjects :
- ENZYMES
PROTEINS
CATALYSTS
HYDRODYNAMICS
BIOCHEMISTRY
Subjects
Details
- Language :
- English
- ISSN :
- 00142956
- Volume :
- 23
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- European Journal of Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 13476008
- Full Text :
- https://doi.org/10.1111/j.1432-1033.1971.tb01618.x