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Identification of the multivalent PDZ protein PDZK1 as a binding partner of sodium-coupled monocarboxylate transporter SMCT1 (SLC5A8) and SMCT2 (SLC5A12).
- Source :
- Journal of Physiological Sciences; Mar2019, Vol. 69 Issue 2, p399-408, 10p
- Publication Year :
- 2019
-
Abstract
- Sodium-coupled monocarboxylate transporters SMCT1 (SLC5A8) and SMCT2 (SLC5A12) mediate the high- and low-affinity transport of lactate in the kidney, but their regulatory mechanism is still unknown. Since these two transporters have the PDZ-motif at their C-terminus, the function of SMCTs may be modulated by a protein-protein interaction. To investigate the binding partner(s) of SMCTs in the kidney, we performed yeast two-hybrid (Y2H) screenings of a human kidney cDNA library with the C-terminus of SMCT1 (SMCT1-CT) and SMCT2 (SMCT2-CT) as bait. PDZK1 was identified as a partner for SMCTs. PDZK1 coexpression in SMCT1-expressing HEK293 cells enhanced their nicotinate transport activity. PDZK1, SMCT1, and URAT1 in vitro assembled into a single tri-molecular complex and their colocalization was confirmed in the renal proximal tubule in vivo by immunohistochemistry. These results indicate that the SMCT1-PDZK1 interaction thus plays an important role in both lactate handling as well as urate reabsorption in the human kidney. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 18806546
- Volume :
- 69
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Journal of Physiological Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 134695056
- Full Text :
- https://doi.org/10.1007/s12576-018-00658-1