A water-soluble BODIPY based ‘OFF/ON’ fluorescent probe for the detection of Cd2+ ions with high selectivity and sensitivity.

A water-soluble dilithium salt BODIPY derivative (LiBDP) with appended dicarboxylate pseudo-crown ether [NO₄] coordinating sites has been designed, synthesized and characterized successfully for the selective and sensitive recognition of Cd²⁺ in aqueous media. The chemosensor exhibits a remarkable increase in fluorescence intensity as well as a distinct color change upon the addition of Cd²⁺ over other environmentally and biologically relevant metal ions in H₂O. The fluorometric response of LiBDP is attributed to the metal chelation-enhanced fluorescence (MCHEF) effect which has been confirmed by a strong association constant of 2.57 ± 1.06 × 10⁵ M⁻¹ and Job's plot, indicating 1 : 1 binding stoichiometry between LiBDP and Cd²⁺. Frontier molecular orbital analysis (obtained from DFT studies) also illustrates the turn-on fluorescence of the probe by blocking photoinduced electron transfer (PET) after coordination to Cd²⁺. The probe can detect Cd²⁺ in a competitive environment up to a submicromolar level in a biologically significant pH range. The sensor is proved to be reversible and reusable by the alternative addition of Cd²⁺ followed by S²⁻. The OFF/ON/OFF sensing behavior is utilized to construct an INHIBIT molecular logic gate based on the two inputs of Cd²⁺ and S²⁻ and a fluorescence intensity at 512 nm as an output. The test paper experiment demonstrates the practical utility of LiBDP to monitor Cd²⁺ in an aqueous sample. Finally, the sensing probe was utilized to monitor Cd²⁺ in living cells. [ABSTRACT FROM AUTHOR]