Back to Search
Start Over
Chromatography of 32P-Labelled Oligonucleotides on Thin Layers of DEAE-Cellulose.
- Source :
- European Journal of Biochemistry; 1969, Vol. 11 Issue 2, p395-399, 5p
- Publication Year :
- 1969
-
Abstract
- A two-dimensional fractionation procedure has been developed fro separating radioactively-labelled oligonucleotides of up to 50 residues long, using uniformly <superscript>32</superscript>P-labelled 5S RNA of Escherichia coli as a model compound. The method uses ionophoresis on cellulose acetate of pH 3.5 in the frist dimension; and ascending chromatography with a concentrated mixture of oligonucleotides on thin layers of mixed DEAE-cellulose and cellulose in the second dimension. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00142956
- Volume :
- 11
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- European Journal of Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 13441931
- Full Text :
- https://doi.org/10.1111/j.1432-1033.1969.tb00786.x