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Endothelial repair is dependent on CD11c+ leukocytes to establish regrowing endothelial sheets with high cellular density.
- Source :
- Journal of Leukocyte Biology; Jan2019, Vol. 105 Issue 1, p195-202, 8p
- Publication Year :
- 2019
-
Abstract
- Endothelial injury makes the vessel wall vulnerable to cardiovascular diseases. Injured endothelium regenerates by collective sheet migration, that is, the endothelial cells coordinate their motion and regrow as a sheet of cells with retained cell‐cell contacts into the wounded area. Leukocytes appear to be involved in endothelial repair in vivo; however, little is known about their identity and role in the reparative sheet migration process. To address these questions, we developed a high‐quality en face technique that enables visualizing of leukocytes and endothelial cells simultaneously following an endoluminal scratch wound injury of the mouse carotid artery. We discovered that regrowing endothelium forms a broad proliferative front accompanied by CD11c+ leukocytes. Functionally, the leukocytes were dispensable for the initial migratory response of the regrowing endothelial sheet, but critical for the subsequent formation and maintenance of a front zone with high cellular density. Marker expression analyses, genetic fate mapping, phagocyte targeting experiments, and mouse knock‐out experiments indicate that the CD11c+ leukocytes were mononuclear phagocytes with an origin from both Ly6Chigh and Ly6Clow monocytes. In conclusion, CD11c+ mononuclear phagocytes are essential for a proper endothelial regrowth following arterial endoluminal scratch injury. Promoting the endothelial‐preserving function of CD11c+ leukocytes may be a strategy to enhance endothelial repair following surgical and endovascular procedures. CD11c+ mononuclear phagocytes are recruited specifically to regenerating endothelium following vascular injury to orchestrate endothelial sheet migration. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 07415400
- Volume :
- 105
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Journal of Leukocyte Biology
- Publication Type :
- Academic Journal
- Accession number :
- 133754774
- Full Text :
- https://doi.org/10.1002/JLB.4A1017-402RR