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Human serum albumin templated MnO2 nanosheets are oxidase mimics for colorimetric determination of hydrogen peroxide and for enzymatic determination of glucose.
- Source :
- Microchimica Acta; Dec2018, Vol. 185 Issue 12, p1-1, 1p
- Publication Year :
- 2018
-
Abstract
- This paper reports on a colorimetric assay for H<subscript>2</subscript>O<subscript>2</subscript> and glucose. It is based on the use of human serum albumin-templated MnO<subscript>2</subscript> nanosheets that possess oxidase-like activity. They are capable of oxidizing 3,3',5,5'-tetramethylbenzidine (TMB) with oxygen to give a blue product (oxTMB) with an absorbance maximum at 652 nm. When H<subscript>2</subscript>O<subscript>2</subscript> is introduced, the MnO<subscript>2</subscript> nanosheets are reduced to Mn(II) ions, and this inhibits the formation of oxTMB. Based on these findings, a colorimetric assay was established for H<subscript>2</subscript>O<subscript>2</subscript> that has a 0.56 μM detection limit. If glucose is oxidized by glucose oxidase under formation of H<subscript>2</subscript>O<subscript>2</subscript>, the nanosheets can be used to quantify H<subscript>2</subscript>O<subscript>2</subscript> and thereby to sense glucose. Response is linear in the 0.5 μM to 50 μM glucose concentration range, and the detection limit is 0.32 μM. The method was applied to the determination of glucose in spiked serum samples and gave satisficatory results.Human serum albumin (HSA) is used as a template for the synthesis of MnO<subscript>2</subscript> nanosheet. These possess oxidase mimicking activity. H<subscript>2</subscript>O<subscript>2</subscript> can reduce the nanosheets. The effect is exploited in colorimetric assays for H<subscript>2</subscript>O<subscript>2</subscript> and glucose using tetramethylbenzidine (TMB) as a chromogenic substrate. [ABSTRACT FROM AUTHOR]
- Subjects :
- DETECTION limit
COLORIMETRIC analysis
GLUCOSE oxidase
ANALYTICAL chemistry
OXIDASES
Subjects
Details
- Language :
- English
- ISSN :
- 00263672
- Volume :
- 185
- Issue :
- 12
- Database :
- Complementary Index
- Journal :
- Microchimica Acta
- Publication Type :
- Academic Journal
- Accession number :
- 133714392
- Full Text :
- https://doi.org/10.1007/s00604-018-3099-5