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Circulating MicroRNA-4739 May Be a Potential Biomarker of Critical Limb Ischemia in Patients with Diabetes.
- Source :
- BioMed Research International; 11/14/2018, p1-8, 8p
- Publication Year :
- 2018
-
Abstract
- Critical limb ischemia (CLI) is the most severe manifestation of peripheral artery disease, which is common but rarely diagnosed. Noninvasive biomarkers are urgently required to assist in the diagnosis of CLI. Accumulating evidence indicates that miRNAs play an important role in the development of various diseases. In this study, microarray profiling revealed 11 miRNAs with significantly altered expression in four T2DM patients with CLI compared with that in four sex- and age-matched T2DM patients without CLI. In independent cohorts, qRT-PCR validation confirmed the increased miRNA-4739 level in patients with CLI versus patients without CLI. miRNA-4739 levels increased with FPG and HbA1c (all P < 0.05). After adjusting for the risk factors, miRNA-4739 levels were found to be associated with an increased odds ratio (OR) of T2DM with CLI (OR =12.818, 95% confidence intervals (CI) 1.148 to 143.143, P = 0.038). ROC curve analysis revealed that the area under the curve (AUC) of miR-4739+confounding risk factors was 0.94 (95% CI 0.891 to 0.998, P < 0.001), which was higher than that of confounding risk factors (AUC 0.94 vs. 0.91, 95% CI -0.122 to 0.060, P > 0.05) and of miR-4739 (AUC 0.94 vs. 0.69, 95% CI -0.399 to -0.101, P < 0.001), respectively. We conclude that elevated plasma miRNA-4739 levels are independently associated with CLI in T2DM patients. miRNA-4739 is implicated as a novel diagnostic marker and a potential therapeutic target for CLI in diabetes. [ABSTRACT FROM AUTHOR]
- Subjects :
- ISCHEMIA diagnosis
ISCHEMIA
PERIPHERAL vascular disease diagnosis
PERIPHERAL vascular diseases
TYPE 2 diabetes complications
AGE distribution
BIOMARKERS
BLOOD sugar
CONFIDENCE intervals
PEOPLE with diabetes
GENE expression
GLYCOSYLATED hemoglobin
POLYMERASE chain reaction
SEX distribution
RECEIVER operating characteristic curves
REVERSE transcriptase polymerase chain reaction
MICROARRAY technology
MICRORNA
ODDS ratio
DISEASE risk factors
Subjects
Details
- Language :
- English
- ISSN :
- 23146133
- Database :
- Complementary Index
- Journal :
- BioMed Research International
- Publication Type :
- Academic Journal
- Accession number :
- 133007724
- Full Text :
- https://doi.org/10.1155/2018/4232794