กำรคัดแยกโปรไบโอติกแบคทีเรียที่มีควำมสำมำรถในกำรยับยั้งเชื้อ Vibrio harveyi และ Vibrio parahaemolyticus ในกุ้งก้ำมกรำม (Macrobrachium rosenbergii, de Man)

Isolation of bacterial strains from the gastrointestinal tract by 45 samples giant freshwater prawns weighing 30-40 grams, found bacteria in 327 isolates. These were screened for antibacterial activity against shrimp pathogenic Vibrio harveyi and Vibrio parahaemolyticus by the clear zone around the colonies. 118 and 19 bacterial isolates, were found the antibacterial activity against pathogenic Vibrio harveyi and Vibrio parahaemolyticus, respectively. The results demonstrated that highest antibacterial activity was shown by seven strains, named PBKS289, PBKS292, PBKS92, PBKS10, PBKS304 and PBKS91. These bacteria were PBKS289, PBKS292, PBKS184 PBKS92, PBKS10, PBKS304 and PBKS91 grew well in a medium containing 0-5% (w/v) bile salt 0-9% NaCl (w/v) and pH 5-10 except PBKS92 could tolerate pH 3-10. The reults support the approach of screening using probiotic bacteria for activity against Vibrio species. The probiotics have a good effect on survival when used as a food in prawn culture. Moreover probiotic bacteria can reduce the need to use toxic chemicals and antibiotics in prawn culture. [ABSTRACT FROM AUTHOR]