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Novel endocrine therapeutic strategy in endometrial carcinoma targeting estrogen-related receptor α by XCT790 and siRNA.
- Source :
- Cancer Management & Research; Aug2018, Vol. 10, p2521-2535, 15p
- Publication Year :
- 2018
-
Abstract
- Purpose: To explore the targeted therapy of estrogen-related receptor α (ERRα) in endometrial cancer (EC) cells and its potential mechanisms.Methods: The mRNA and protein expression levels of ERRα and estrogen receptor α (ERα) were detected by qPCR and Western blotting in RL-952, AN3-CA, HEC-1A, and HEC-1B EC cell lines. After treatment with the ERRα-specific antagonist XCT790 or infection with lentivirus-mediated small interfering RNA (siRNA) targeting the ERRα (siRNA-ERRα), cell proliferation and apoptosis were evaluated by MTS assay and flow cytometry. After treatment with siRNA-ERRα, the expression profiles of transcription factors (TFs) were analyzed by protein/DNA arrays in EC cells.Results: The relative mRNA levels of ERRa in RL-952 (1±0.0831) and AN3-CA (1.162±0.0325) were significantly higher than those in HEC-1A (0.3081±0.0339) and HEC-1B (0.1119±0.0091) (P [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 11791322
- Volume :
- 10
- Database :
- Complementary Index
- Journal :
- Cancer Management & Research
- Publication Type :
- Academic Journal
- Accession number :
- 132657809
- Full Text :
- https://doi.org/10.2147/CMAR.S168043