Effects of fibrin‐binding oligopeptide derived from fibronectin on osteoblast‐like cell migration in in vitro wound healing model.

Background: Objectives of the study‐ to examine the effect of synthetic FN fragments including fibrin binding sites on migration of osteoblast‐like cells via in vitro scratch assay. Aim/Hypothesis: Fibrin‐binding oligopeptides, 100 μM of FF3 and FF5, derived from FN enhanced the migration rate of MC3T3‐E1 osteoblast‐like cells in in vitro wound healing model and these peptides can be applied to enhance the early wound healing capacity of biomaterials inserted into a bone. Material and Methods: MC3T3‐E1 osteoblast‐like cells Synthetic oligopeptide FF1 (CYDNGKHYQ), FF3 (CFDKYTGNTYR), FF5 (CTSRNRCNDQ) ‐ diluted to 50, 100 and 150 μM ‐ coated to 4‐well cell culture plate Scratch wound assay ‐ 1 × 10_SP_5_sp_ cells well ‐ parallel scratches with 200 μl plastic pipette tips ‐ pretreated with mitomycin C (30 μg mL) 1 hour before wounding Fixation and Staining ‐ 14, 20 hours after wounding with Multiple stain Migration rate (%) = area of migrated cells (A) cell free area (A+B). Results: All peptide groups except 150 μM showed statistically significant increase in migration rate at 14 hours. At 20 hours all experimental groups of 100 μM and FF1 of 150 μM concentration were significantly improved the scratch closure. Conclusions and Clinical Implications: Significantly increased migration was observed at type I repeats 2–5 fibrin‐binding fragments of FN – FF3 and FF5 ‐ added MC3T3‐E1 cells. Optimum concentration was 100 μM. The biologic effect of fibrin‐binding oligopeptide in this study supports the FN's essential contribution in bone regeneration as a temporary scaffold in FN‐fibrin matrix. [ABSTRACT FROM AUTHOR]