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Physiological and Regulatory Characterization of KatA and KatY in Yersinia pestis.
- Source :
- DNA & Cell Biology; Aug2008, Vol. 27 Issue 8, p453-462, 10p, 2 Black and White Photographs, 3 Charts, 4 Graphs
- Publication Year :
- 2008
-
Abstract
- The catalase or catalase-peroxidase activity commonly exists in many pathogens and plays an important role in resisting the oxidative burst of phagocytes helping the pathogen persistently colonize in the host. Yersinia pestis is a facultative pathogen and the causative agent of plague. KatY has been identified as a thermosensing antigen with modest catalase activity in this pathogen. Here Y. pestis KatA and KatY were experimentally confirmed as a monofunctional catalase and bifunctional catalase-peroxidase, respectively. Their expression induced by H<subscript>2</subscript>O<subscript>2</subscript> was proven to be mediated by the oxidative regulator, OxyR. Expression of KatA changed with growth phases and was crucial to its traditional physiological role in protecting Y. pestis cells against toxicity of exogenous H<subscript>2</subscript>O<subscript>2</subscript>. KatY was regulated by temperature and H<subscript>2</subscript>O<subscript>2</subscript>, two major elements of phagolysosomal microenvironments. Consistent with the above results, gene expression of katY increased significantly during intracellular growth of Y. pestis compared with that in vitro growth. However, a ΔkatY mutant was fully virulent to mice, suggesting that KatY is not required for Y. pestis virulence. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10445498
- Volume :
- 27
- Issue :
- 8
- Database :
- Complementary Index
- Journal :
- DNA & Cell Biology
- Publication Type :
- Academic Journal
- Accession number :
- 131920767
- Full Text :
- https://doi.org/10.1089/dna.2007.0657