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Poly(styrene-4-sulfonate)-protected copper nanoclusters as a fluorometric probe for sequential detection of cytochrome c and trypsin.
- Source :
- Microchimica Acta; Aug2018, Vol. 185 Issue 8, p1-1, 1p
- Publication Year :
- 2018
-
Abstract
- Stable copper nanoclusters (CuNCs) were prepared by utilizing D-penicillamine as both the stabilizer and reductant. The emission of the CuNCs (with excitation/emission peaks at 390/645 nm) is largely stabilized by coating with poly(sodium-p-styrenesulfonate) (PSS). Cytochrome c (Cyt c) quenches the fluorescence of the PSS-coated CuNCs, and this effect was exploited to design a quenchometric fluorometric assay for Cyt c. If trypsin is added to the loaded CuNCs, it will hydrolyze Cyt c to form peptide fragments, and fluorescence is gradually restored. A highly sensitive and fluorometric turn-off-on assay was constructed for sequential detection of Cyt c and trypsin. The linear ranges for Cyt c and trypsin are from 8.0 nM to 680 nM, and from 0.1 to 6.0 μg mL<superscript>−1</superscript>, and the lower detection limits are 0.83 nM and 20 ng mL<superscript>−1</superscript> for Cyt c and trypsin, respectively.Schematic illustration of the fluorometric assay for trypsin based on the electron transfer between poly(p-styrenesulfonate)-protected copper nanoclusters (PSS-CuNCs) and cytochrome c (Cyt c).<graphic></graphic> [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00263672
- Volume :
- 185
- Issue :
- 8
- Database :
- Complementary Index
- Journal :
- Microchimica Acta
- Publication Type :
- Academic Journal
- Accession number :
- 131336082
- Full Text :
- https://doi.org/10.1007/s00604-018-2920-5