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MiR-1256 suppresses proliferation and migration of non-small cell lung cancer via regulating TCTN1.
- Source :
- Oncology Letters; Aug2018, Vol. 16 Issue 2, p1708-1714, 7p
- Publication Year :
- 2018
-
Abstract
- Mounting evidence has shown that miRNA expression is abnormal in various human cancers. Here, we mainly explored the biological function and the potential mechanisms of miR-1256 in non-small cell lung cancer (NSCLC). The miR-1256 mRNA expression was detected by quantitative real-time PCR and tectonic family member 1 (TCTN1) mRNA expression was detected by immunoblotting. The TCTN1 was identified to be the direct and specific target gene of miR-1256 by luciferase reporter assay. Cell proliferation was examined by methyl thiazolyl tetrazolium assay and migration was detected by transwell assay. MiR-1256 expression was downregulated in NSCLC tissues, whereas the expression of TCTN1 was upregulated, compared with normal tissues. We also found that overexpression of miR-1256 in these NSCLC cell lines inhibited cell proliferation and migration. Furthermore, TCTN1 was identified as a direct target of miR-1256 by luciferase reporter assays. Collectively, these data stated that the inhibitory effect of miR-1256 in NSCLC was realized by upregulating TCTN1, suggesting that miR-1256/TCTN1 axis may play a critical role as NSCLC therapeutic target. [ABSTRACT FROM AUTHOR]
- Subjects :
- MICRORNA
NON-small-cell lung carcinoma
CELL proliferation
GENE expression
APOPTOSIS
Subjects
Details
- Language :
- English
- ISSN :
- 17921074
- Volume :
- 16
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Oncology Letters
- Publication Type :
- Academic Journal
- Accession number :
- 130697939
- Full Text :
- https://doi.org/10.3892/ol.2018.8794