Determination of Multiple Pesticide Residues in Okra by QuEChERS Combined with PTV-GC-MS/MS and UPLC-MS/MS.

[Objectives] To establish QuEChERS based extraction method for determining multiple pesticide residues in okra combined with programmed temperature vaporizer-gas chromatography-triple quadrupole mass spectrometry (PTV-GC-MS/MS) and ultra high performance liquid chromatography-mass spectrometry (UPLC-MS/MS). [Methods] In this experiment, 124 pesticide residues in okra were determined by an optimized QuEChERS method combined with PTV-GC-MS/MS and UPLC-MS/MS. The pesticides in okra were extracted and purified by the optimized QuEChERS pretreatment method and determined by PTV-GC-MS/MS and UPLC-MS/MS. [ Results] In this experiment, 124 kinds of pesticides had a good linearity with a limit of detection (LOD) of 0.000 5 - 0.008 0 mg/kg; the average recovery rate at three levels was 65.5% - 128.1% with relative standard deviation of 2.2% -9.7%. [Conclusions] This method is simple, rapid, accurate and sensitive, and can provide reliable and valid data support for the determination of multiple pesticide residues in okra by GC-MS/MS and UPLC-MS/MS. [ABSTRACT FROM AUTHOR]