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Hydroxylated-Graphene Quantum Dots Induce DNA Damage and Disrupt Microtubule Structure in Human Esophageal Epithelial Cells.
- Source :
- Toxicological Sciences; Jul2018, Vol. 164 Issue 1, p339-352, 14p, 4 Diagrams, 3 Charts, 3 Graphs
- Publication Year :
- 2018
-
Abstract
- Graphene quantum dots (GQDs) have attracted significant interests due to their unique chemical and physical properties. In this study, we investigated the potential effects of hydroxyl-modified GQDs (OH-GQDs) on the human esophageal epithelial cell line HET-1A. Our data revealed significant cytotoxicity of OH-GQDs which decreased the viability of HET-1A in a dose and time-dependent manner. The moderate concentration (25 or 50 µg/ml) of OH-GQDs significantly blocked HET-1A cells in G0/G1 cell cycle phase. An increased percentage of γH2AX-positive and genomically unstable cells were also detected in cells treated with different doses of OH-GQDs (25, 50, and 100 µg/ml). Microarray data revealed that OH-GQDs treatment down-regulated genes related to DNA damage repair, cell cycle regulation and cytoskeleton signal pathways indicating a novel role of OH-GQDs. Consistent with the microarray data, OH-GQDs disrupted microtubule structure and inhibited microtubule regrowth around centrosomes in HET-1A cells. In conclusion, our findings provide important evidence for considering the application of OH-GQDs in biomedical fields. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10966080
- Volume :
- 164
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Toxicological Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 130354130
- Full Text :
- https://doi.org/10.1093/toxsci/kfy090