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The yeast non-Mendelian factor [ETA+] is a variant of [PSI+], a prion-like form of release factor eRF3.
- Source :
- EMBO Journal; 3/1/99, Vol. 18 Issue 5, p1182-1191, 10p
- Publication Year :
- 1999
-
Abstract
- The yeast non­Mendelian factor [ETA<superscript>+</superscript>] is lethal in the presence of certain mutations in the SUP35 and SUP45 genes, which code for the translational release factors eRF3 and eRF1, respectively. One such mutation, sup35-2, is now shown to contain a UAG stop codon prior to the essential region of the gene. The non­Mendelian inheritance of [ETA<superscript>+</superscript>] is reminiscent of the yeast [PSI<superscript>+</superscript>] element, which is due to a self-propagating conformation of Sup35p. Here we show that [ETA<superscript>+</superscript>] and [PSI<superscript>+</superscript>] share many characteristics. Indeed, like [PSI<superscript>+</superscript>], the maintenance of [ETA<superscript>+</superscript>] requires the N-terminal region of Sup35p and depends on an appropriate level of the chaperone protein Hsp104. Moreover, [ETA<superscript>+</superscript>] can be induced de novo by excess Sup35p, and [ETA<superscript>+</superscript>] cells have a weak nonsense suppressor phenotype characteristic of weak [PSI<superscript>+</superscript>]. We conclude that [ETA ] is actually a weak, unstable variant of [PSI ]. We find that although some Sup35p aggregates in [ETA<superscript>+</superscript>] cells, more Sup35p remains soluble in [ETA<superscript>+</superscript>] cells than in isogenic strong [PSI<superscript>+</superscript>] cells. Our data suggest that the amount of soluble Sup35p determines the strength of translational non-sense suppression associated with different [PSI<superscript>+</superscript>] variants. [ABSTRACT FROM AUTHOR]
- Subjects :
- GENETIC mutation
SUPPRESSOR cells
GENES
HEREDITY
GENETICS
PROTEINS
MOLECULAR biology
Subjects
Details
- Language :
- English
- ISSN :
- 02614189
- Volume :
- 18
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- EMBO Journal
- Publication Type :
- Academic Journal
- Accession number :
- 13003912
- Full Text :
- https://doi.org/10.1093/emboj/18.5.1182