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Diverse transcriptional initiation revealed by fine, large-scale mapping of mRNA start sites.
- Source :
- EMBO Reports; May2001, Vol. 2 Issue 5, p388-393, 6p
- Publication Year :
- 2001
-
Abstract
- Determination of the mRNA start site is the first step in identifying the promoter region, which is of key importance for transcriptional regulation of gene expression. The 'oligo-capping' method enabled us to introduce a sequence tag to the first base of an mRNA by replacing the cap structure of the mRNA. Using cDNA libraries made from oligo-capped mRNAs, we could identify the transcriptional start site of an individual mRNA just by sequencing the 5'-end of the cDNA. The fine mapping of transcriptional start sites was performed for 5880 mRNAs in 276 human genes. Contrary to our expectations, the majority of the genes showed a diverse distribution of transcriptional start sites. They were distributed over 61.7 bp with a standard deviation of 19.5. Our finding may reflect the dynamic nature of transcriptional initiation events of human genes in vivo. [ABSTRACT FROM AUTHOR]
- Subjects :
- MESSENGER RNA
GENE expression
GENETIC regulation
GENE mapping
DNA
GENES
Subjects
Details
- Language :
- English
- ISSN :
- 1469221X
- Volume :
- 2
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- EMBO Reports
- Publication Type :
- Academic Journal
- Accession number :
- 12956780
- Full Text :
- https://doi.org/10.1093/embo-reports/kve085