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The PACT domain, a conserved centrosomal targeting motif in the coiled-coil proteins AKAP450 and pericentrin.
- Source :
- EMBO Reports; Dec2000, Vol. 1 Issue 6, p524-529, 6p
- Publication Year :
- 2000
-
Abstract
- AKAP450 (also known as AKAP350, CG-NAP or Hyperion) and pericentrin are large coiled-coil proteins found in mammalian centrosomes that serve to recruit structural and regulatory components including dynein and protein kinase A. We find that these proteins share a well conserved 90 amino acid domain near their C-termini that is also found in coiled-coil proteins of unknown function from Drosophila and fission yeast. Fusion of the C-terminal region from either protein to a reporter protein confers a centrosomal localization, and over- expression of the domain from AKAP450 displaces endogenous pericentrin, suggesting recruitment to a shared site. When isolated from transfected cells the C-terminal domain of AKAP450 was associated with calmodulin, suggesting that this protein could contribute to centrosome assembly. [ABSTRACT FROM AUTHOR]
- Subjects :
- PROTEINS
CELLS
PROTEIN kinases
AMINO acids
ORGANIC acids
YEAST
Subjects
Details
- Language :
- English
- ISSN :
- 1469221X
- Volume :
- 1
- Issue :
- 6
- Database :
- Complementary Index
- Journal :
- EMBO Reports
- Publication Type :
- Academic Journal
- Accession number :
- 12956676
- Full Text :
- https://doi.org/10.1093/embo-reports/kvd105