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<bold><italic>Euodia daniellii</italic></bold> Hemsl. (Bee-Bee Tree) Oil Attenuates Palmitate-Induced Lipid Accumulation and Apoptosis in Hepatocytes.
- Source :
- Pharmacology; Apr2018, Vol. 101 Issue 5/6, p298-308, 11p, 6 Graphs
- Publication Year :
- 2018
-
Abstract
- Hepatic lipid accumulation and apoptosis is elevated in patients with non-alcoholic steatohepatitis and is closely associated with severity. Saturated fatty acid palmitate stimulates lipid accumulation and apoptosis in hepatocytes. In the present study, we examined bee-bee tree oil (BO)-mediated protective effects on palmitate-induced lipid accumulation and apoptosis in mouse primary hepatocytes. Cells were cultured in a control media or the same media containing 150 or 300 µmol/L of albumin-bound palmitate for 24 h. BO concentrations used were 0, 0.1, 0.2, or 0.5%. Palmitate induced lipid accumulation and mRNA expression of lipogenic genes such as <italic>SREBP1c</italic> and <italic>SCD1</italic>. However, BO prevented these changes. Furthermore, palmitate stimulated caspase-3 activity and decreased cell viability in the absence of BO. BO reduced palmitate-induced activation of caspase-3 and cell death in a dose-dependent manner. AMP-activated protein kinase inhibitors abolished the effects of BO. Furthermore, BO suppressed palmitate-induced c-Jun N-terminal kinase (JNK) phosphorylation through the 5' adenosine monophosphate-activated protein kinase (AMPK)-dependent pathway. In conclusion, BO attenuated palmitate-induced hepatic steatosis and apoptosis through AMPK-mediated suppression of JNK signaling. These data suggest that BO is an important determinant of saturated fatty acid-induced lipid accumulation and apoptosis, and may be an effective therapeutic strategy for treatment of obesity-mediated liver diseases. [ABSTRACT FROM AUTHOR]
- Subjects :
- FATTY degeneration
BLOOD lipids
HYPERLIPIDEMIA
LIPID synthesis
ENDOPLASMIC reticulum
Subjects
Details
- Language :
- English
- ISSN :
- 00317012
- Volume :
- 101
- Issue :
- 5/6
- Database :
- Complementary Index
- Journal :
- Pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 129340003
- Full Text :
- https://doi.org/10.1159/000487892