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Effect of Total Flavone of Haw Leaves on Nuclear Factor Erythroid-2 Related Factor and Other Related Factors in Nonalcoholic Steatohepatitis Rats.
- Source :
- Chinese Journal of Integrative Medicine; Apr2018, Vol. 24 Issue 4, p265-271, 7p
- Publication Year :
- 2018
-
Abstract
- Objective: To investigate the effect of total flavone of haw leaves (TFHL) on the expression of nuclear factor erythroid-2 related factor (Nrf2) and other related factors in nonalcoholic steatohepatitis (NASH) rats induced by high-fat diet and then to further discuss the mechanism of TFHL’s prevention against NASH.Methods: High-fat diet was fed to 40 rats to establish the NASH model. Then model rats were intragastrically administrated with 40, 80, 160 mg/(kg•day) TFHL, respectively. The pathological changes of liver tissues in NASH rats were detected by oil red O and hematoxylin-eosin (HE) stainings. The expression of Nrf2 in rat liver was examined through immunohistochemistry. The level of 8-iso-prostaglandin F2α in serum was detected through enzyme linked immunosorbent assay (ELISA). The mRNA and protein levels of Nrf2 and other related factors in liver tissue were measured by real-time reverse transcriptionpolymerase chain reaction and western blot.Results: Lipid deposition, hepatic steatosis, focal necrosis in lobular inflammation and ballooning degeneration were emerged in livers of NASH rats. The 8-iso-prostaglandin F2α in the serum of NASH rats increased significantly compared with the control group (<italic>P</italic><0.05). The mRNA of Nrf2, hemeoxyenase1 (HO-1) and the mRNA and protein levels of quinine oxidoreductase (NQO1) in NASH rats liver tissue showed a striking increase, while the mRNA levels of Keap1, r-glutamylcysteine synthethase (rGCS) and glutathione S-transferase (GST) were significantly decreased compared with the control group (<italic>P</italic><0.05). After TFHL treatment, 8-iso-prostaglandin F2α level in serum significantly decreased, and Nrf2 mRNA and protein levels in hepatocytes nucleus enhanced compared with the model group (<italic>P</italic><0.05 or 0.01). Meanwhile the Keap1 mRNA, the mRNA and protein levels of HO-1, NQO1 antibody, rGCS antibody, GST increased after TFHL treatment (<italic>P</italic><0.05 or 0.01).Conclusions: Nrf2 and other related factors were involved in development of NASH, and they also served as an important part in its occurrence. By regulating expression of Nrf2 and other related factors, TFHL may play a role in antioxidative stress and prevention of NASH. [ABSTRACT FROM AUTHOR]
- Subjects :
- FATTY liver prevention
ANIMAL experimentation
ENZYME-linked immunosorbent assay
FATTY liver
FAT content of food
GENE expression
GLUTATHIONE
IMMUNOHISTOCHEMISTRY
INFLAMMATORY mediators
LEAVES
OXIDOREDUCTASES
POLYMERASE chain reaction
PROSTAGLANDINS
QUININE
RATS
STAINS & staining (Microscopy)
TRANSCRIPTION factors
WESTERN immunoblotting
OXIDATIVE stress
REVERSE transcriptase polymerase chain reaction
FLAVONES
PHARMACODYNAMICS
Subjects
Details
- Language :
- English
- ISSN :
- 16720415
- Volume :
- 24
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Chinese Journal of Integrative Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 129302274
- Full Text :
- https://doi.org/10.1007/s11655-016-2450-0