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DNAzyme–aptamer or aptamer–DNAzyme paradigm: Biochemical approach for aflatoxin analysis.
- Source :
- Biotechnology & Applied Biochemistry; Mar/Apr2018, Vol. 65 Issue 2, p274-280, 7p
- Publication Year :
- 2018
-
Abstract
- Abstract: DNAzyme and aptamer conjugations have already been used for sensitive and accurate detection of several molecules. In this study, we tested the relationship between conjugation orientation of DNAzyme and aflatoxin B1 aptamer and their subsequent peroxidase activity. Circular dichroism (CD) spectroscopy and biochemical analysis were used here to differentiate between these two conjugation patterns. Results showed that DNAzyme–aptamer has more catalytic activity and efficiency than aptamer–DNAzyme. Thereby, DNAzyme–aptamer with its superior efficiency can be used for design and development of more sensitive aflatoxin B1 DNA based biosensors. [ABSTRACT FROM AUTHOR]
- Subjects :
- DNA
ENZYMES
APTAMERS
AFLATOXIN genetics
PEROXIDASE
Subjects
Details
- Language :
- English
- ISSN :
- 08854513
- Volume :
- 65
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Biotechnology & Applied Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 129104481
- Full Text :
- https://doi.org/10.1002/bab.1563