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Methotrexate-conjugated L-lysine coated iron oxide magnetic nanoparticles for inhibition of MCF-7 breast cancer cells.
- Source :
- Drug Development & Industrial Pharmacy; Jun2018, Vol. 44 Issue 6, p886-894, 9p
- Publication Year :
- 2018
-
Abstract
- Methotrexate (MTX), a stoichiometric inhibitor of dihydrofolate reductase enzyme, is a chemotherapeutic agent for treating a diversity of neoplasms. In this study, we design and developed a new formulation of MTX that serves as drug carrier and examined its cytotoxic effect <italic>in vitro</italic>. This target drug delivery system is dependent on the release of the MTX within the lysosomal compartment. The iron oxide magnetic nanoparticles (IONPs) were first surface-coated with L-lysine and subsequently conjugated with MTX through amidation between the carboxylic acid end groups on MTX and the amine groups on the IONPs surface. MTX-conjugated L-lysine coated IONPs (F-Lys-MTX NPs) was characterized by X-ray diffraction, thermogravimetric analysis, differential scanning calorimetry, Fourier transform infrared spectroscopy, vibrating sample magnetometer, and transmission electron microscopy techniques. The cytotoxicity of the void of MTX and F-Lys-MTX NPs were compared to each other by MTT assay of the treated MCF-7 cell lines. The results showed that the ζ-potential of F-Lys-MTX NPs was about −5.49 mV and the average size was 43.72 ± 4.73 nm. Model studies exhibited the release of MTX via peptide bond cleavage in the presence of proteinase K and at low pH. These studies specify that F-Lys-MTX NPs have a very remarkable anticancer effect, for breast cancer cell lines. [ABSTRACT FROM AUTHOR]
- Subjects :
- METHOTREXATE
LYSINE
FERRIC oxide
BREAST cancer
NANOPARTICLES
Subjects
Details
- Language :
- English
- ISSN :
- 03639045
- Volume :
- 44
- Issue :
- 6
- Database :
- Complementary Index
- Journal :
- Drug Development & Industrial Pharmacy
- Publication Type :
- Academic Journal
- Accession number :
- 128814856
- Full Text :
- https://doi.org/10.1080/03639045.2017.1417422