Pathologic and biochemical characterization of PrPSc from elk with <italic>PRNP</italic> polymorphisms at codon 132 after experimental infection with the chronic wasting disease agent.

Background: The Rocky Mountain elk (<italic>Cervus elaphus nelsoni</italic>) prion protein gene (<italic>PRNP</italic>) is polymorphic at codon 132, with leucine (L132) and methionine (M132) allelic variants present in the population. In elk experimentally inoculated with the chronic wasting disease (CWD) agent, different incubation periods are associated with <italic>PRNP</italic> genotype: LL132 elk survive the longest, LM132 elk are intermediate, and MM132 elk the shortest. The purpose of this study was to investigate potential mechanisms underlying variations in incubation period in elk of different prion protein genotypes. Elk calves of three <italic>PRNP</italic> genotypes (<italic>n</italic> = 2 MM132, <italic>n</italic> = 2 LM132, <italic>n</italic> = 4 LL132) were orally inoculated with brain homogenate from elk clinically affected with CWD. Results: Elk with longer incubation periods accumulated relatively less PrP^Sc in the brain than elk with shorter incubation periods. PrP^Sc accumulation in LM132 and MM132 elk was primarily neuropil-associated while glial-associated immunoreactivity was prominent in LL132 elk. The fibril stability of PrP^Sc from MM132 and LM132 elk were similar to each other and less stable than that from LL132 elk. Real-time quaking induced conversion assays (RT-QuIC) revealed differences in the ability of PrP^Sc seed from elk of different genotypes to convert recombinant 132 M or 132 L substrate. Conclusions: This study provides further evidence of the importance of <italic>PRNP</italic> genotype in the pathogenesis of CWD of elk. The longer incubation periods observed in LL132 elk are associated with PrP^Sc that is more stable and relatively less abundant at the time of clinical disease. The biochemical properties of PrP^Sc from MM132 and LM132 elk are similar to each other and different to PrP^Sc from LL132 elk. The shorter incubation periods in MM132 compared to LM132 elk may be the result of genotype-dependent differences in the efficiency of propagation of PrP^Sc moieties present in the inoculum. A better understanding of the mechanisms by which the polymorphisms at codon 132 in elk <italic>PRNP</italic> influence disease pathogenesis will help to improve control of CWD in captive and free-ranging elk populations. [ABSTRACT FROM AUTHOR]