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Development and Characterization of Solid Lipid Nanoparticles Loaded with a Highly Active Doxorubicin Derivative.
- Source :
- Nanomaterials (2079-4991); Feb2018, Vol. 8 Issue 2, p110, 16p
- Publication Year :
- 2018
-
Abstract
- Solid lipid nanoparticles (SLNs) comprise a versatile drug delivery system that has been developed for the treatment of a variety of diseases. The present study will investigate the feasibility of entrapping an active doxorubicin prodrug (a squalenoyl-derivative) in SLNs. The doxorubicin derivative-loaded SLNs are spherically shaped, have a mean diameter of 300-400 nm and show 85% w/w drug entrapment efficiency. The effects on cell growth of loaded SLNs, free doxorubicin and the prodrug have been examined using cytotoxicity and colony-forming assays in both human ovarian cancer line A2780 wild-type and doxorubicin-resistant cells. Further assessments as to the treatment's ability to induce cell death by apoptosis have been carried out by analyzing annexin-V staining and the activation of caspase-3. The in vitro data demonstrate that the delivery of the squalenoyl-doxorubicin derivative by SLNs increases its cytotoxic activity, as well as its apoptosis effect. This effect was particularly evident in doxorubicin-resistant cells. [ABSTRACT FROM AUTHOR]
- Subjects :
- NANOPARTICLES
DOXORUBICIN
Subjects
Details
- Language :
- English
- ISSN :
- 20794991
- Volume :
- 8
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Nanomaterials (2079-4991)
- Publication Type :
- Academic Journal
- Accession number :
- 128258902
- Full Text :
- https://doi.org/10.3390/nano8020110