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Description of a cryptic thermophilic (pro)phage, CBP1 from <italic>Caldibacillus debilis</italic> strain GB1.

Authors :
Wushke, Scott
Jin, Zimo
Spicer, Victor
Zhang, Xiang Li
Fristensky, Brian
Krokhin, Oleg V.
Levin, David B.
Sparling, Richard
Source :
Extremophiles; Mar2018, Vol. 22 Issue 2, p203-209, 7p
Publication Year :
2018

Abstract

This study characterizes a cryptic (pro)phage-related sequence within the &lt;italic&gt;Caldibacillus debilis&lt;/italic&gt; GB1 genome, designated CBP1.CBP1 is a Siphoviridae-like genome highly related to GBVS1 from &lt;italic&gt;Geobacillus&lt;/italic&gt; sp. 6k51. The CBP1genome is a 37,315&#160;bp region containing 69 putative ORFs with a GC content of 42% flanked on both sides by host DNA integrated into the main bacterial chromosome (contig 16). Bioinformatic analyses identified cassettes of genes within the CBP1 genome that were similar in function, yet distinct in sequence, from genes previously identified in GBVS1. All of CBP1 genes had less than 60% amino acid sequence identity with GBVS1by tBLASTx, with the exception of the TMP repeat gene. CBP1 possessed all the necessary genes to undergo a temperate/lytic phage life cycle, including excision, replication, structural genes, DNA packaging, and cell lyses. Proteomic analysis of CBP1 revealed the expression of 5 proteins. One of the expressed proteins was a transcriptional regulator protein homologous to the bacteriophage λ repressor protein (&lt;italic&gt;cI&lt;/italic&gt;) expressed in high amounts from the CBP1 region, consistent with a lysogenic phage in a repressed state. The CBP1 protein expression profile during host growth provides unique insight into thermophilic &lt;italic&gt;Siphoviridae&lt;/italic&gt;-like phages in the repressed state within their host cells. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
14310651
Volume :
22
Issue :
2
Database :
Complementary Index
Journal :
Extremophiles
Publication Type :
Academic Journal
Accession number :
128186737
Full Text :
https://doi.org/10.1007/s00792-017-0988-1