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Fishing for contaminants: identification of three mechanism specific transcriptome signatures using <italic>Danio rerio</italic> embryos.

Authors :
Hausen, Jonas
Otte, Jens C.
Legradi, Jessica
Yang, Lixin
Strähle, Uwe
Fenske, Martina
Hecker, Markus
Tang, Song
Hammers-Wirtz, Monika
Hollert, Henner
Keiter, Steffen H.
Ottermanns, Richard
Source :
Environmental Science & Pollution Research; Feb2018, Vol. 25 Issue 5, p4023-4036, 14p
Publication Year :
2018

Abstract

In ecotoxicology, transcriptomics is an effective way to detect gene expression changes in response to environmental pollutants. Such changes can be used to identify contaminants or contaminant classes and can be applied as early warning signals for pollution. To do so, it is important to distinguish contaminant-specific transcriptomic changes from genetic alterations due to general stress. Here we present a first step in the identification of contaminant class-specific transcriptome signatures. Embryos of zebrafish (&lt;italic&gt;Danio rerio&lt;/italic&gt;) were exposed to three substances (methylmercury, chlorpyrifos and Aroclor 1254, each from 24 to 48&#160;hpf exposed) representing sediment typical contaminant classes. We analyzed the altered transcriptome to detect discriminative genes significantly regulated in reaction to the three applied contaminants. By comparison of the results of the three contaminants, we identified transcriptome signatures and biologically important pathways (using Cytoscape/ClueGO software) that react significantly to the contaminant classes. This approach increases the chance of finding genes that play an important role in contaminant class-specific pathways rather than more general processes. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
09441344
Volume :
25
Issue :
5
Database :
Complementary Index
Journal :
Environmental Science & Pollution Research
Publication Type :
Academic Journal
Accession number :
128148751
Full Text :
https://doi.org/10.1007/s11356-017-8977-6