The Fractionation of the Histones of Group F2(b) from Calf Thymus.

The histones of group F2(b) have been fractionated by acetone precipitation and by column chromatography on carboxymethylcellulose at pH 9. The acetone precipitation method enables F2(b), prepared on a large scale by extraction methods, to be further purified in bulk, the resulting product having 84% N-terminal proline. The column chromatography on carboxymethylcellulose at pH 9 has given 5 main fractions, two of which contain over 25% acidic amino acids (or their amides) and over 24% basic amino acids. [ABSTRACT FROM AUTHOR]