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Simultaneous determination and pharmacokinetic study of giraldoid a, giraldoid B in rat plasma after oral administration of <italic>Daphne giraldii</italic> Nitsche extracts by LC–MS/MS.
- Source :
- Biomedical Chromatography; Mar2018, Vol. 32 Issue 3, p1-1, 8p
- Publication Year :
- 2018
-
Abstract
- Abstract: A simple sensitive LC–MS/MS method has been developed for the simultaneous determination of giraldoid A and giraldoid B in rat plasma. The method was applied to pharmacokinetics studies of the two compounds from Daphne giraldii Nitsche. Chromatographic separation was accomplished on an Acquity UPLC™ BEH C<subscript>18</subscript> column (100 × 2.1 mm, 1.7 mm) by gradient elution with a flow rate of 0.2 mL min<superscript>−1</superscript><subscript>.</subscript> The method was linear over the concentration range of 1.0–1000 ng mL<superscript>−1</superscript>, and the lower limits of quantification were 1.04 ± 0.10 and 1.04 ± 0.09 ng mL<superscript>−1</superscript>, respectively. The intra‐ and inter‐day precisions (RSD) were <10.14 and 9.96%. The extraction recovery of the analytes was acceptable. Stability studies demonstrated that the two compounds were stable in the preparation and analytical process. The maximum plasma concentration was 687.78 ± 243.62 ng mL<superscript>−1</superscript> for giraldoid A and 952.38 ± 131.99 ng mL<superscript>−1</superscript> for giraldoid B. The time to reach the maximum plasma concentration was 0.50 ± 0.37 h for giraldoid A and 0.50 ± 0.66 h for giraldoid B. The validated method was successfully applied to investigate the concentration–time profiles of giraldoid A and giraldoid B. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 02693879
- Volume :
- 32
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Biomedical Chromatography
- Publication Type :
- Academic Journal
- Accession number :
- 127847613
- Full Text :
- https://doi.org/10.1002/bmc.4129