Isolation and structure determination of a new flavone glycoside from seed residues of seabuckthorn ( Hippophae rhamnoides L.).

In this study, a valid method was established for the isolation and purification of flavone glycosides fromHippophae rhamnoidesL. seed residues using high-speed counter-current chromatography in one step, with a solvent system of ethyl acetate-methanol-n-butyl alcohol-water (9:1:0.5:9,v/v/v/v). A total of 28.8 mg compoundIand 57.3 mg compoundIIwere obtained from 200 mg of flavone H-glycosides rich extract, with purities of 98.3 and 96.4%, respectively. The structures of two compounds were identified by MS and NMR. 3-O-β-D-Sophorosylkaempferol-7-O-{3-O-[2(E)-2,6-dimethyl-6-hydroxyocta-2,7-dienoyl]}-α-L-rhamnoside is compoundIand compoundIInamed hippophanone is a new compound were identified by MS and NMR. The method was efficient and convenient, which could be used for the preparative separation of flavone glycosides fromH. rhamnoidesL. seed residues. [ABSTRACT FROM PUBLISHER]