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Simultaneous Determination of Alpha-Tocopherol and Alpha-Tocopheryl Acetate in Dairy Products, Plant Milks and Health Supplements by Using SPE and HPLC Method.
- Source :
- Food Analytical Methods; Dec2017, Vol. 10 Issue 12, p3886-3901, 16p
- Publication Year :
- 2017
-
Abstract
- A solid phase extraction technique for sample cleanup and extraction of α-tocopherol and α-tocopheryl acetate is described and applied to their simultaneous HPLC analysis in dairy products, plant-based milks, infant formulas, and pharmaceutical colostrum. The proposed method includes sample pretreatment by using ethanol, followed by solid-phase extraction of the organic supernatant on C18 cartridges. The identification and quantification were done by reversed-phase HPLC with fluorescence detection (ex 295 nm, em 330 nm) for tocopherol and with UV detector set at 220 nm for tocopheryl acetate. The mobile phase consisted of 100% acetonitrile. The linear ranges were from 0.02-0.40 μg/mL (dairy products) and 0.50-5.00 μg/mL (plant milks, infant formulas, powdered products) for α-tocopherol and those of α-tocopheryl acetate were from 0.20-2.00 μg/mL (dairy products) and 1.00-10.0 μg/mL (plant milks, infant formulas, powdered products). The LOD and LOQ of α-tocopherol were 0.1 and 0.4 μg/mL, while those of the α-tocopheryl acetate were 2.0 μg/mL for LOD and 6.0 μg/mL for LOQ. The method gave extraction recoveries from 73-94% with RSDs values being 5-10%. This method is simple, SPE procedure is fast and HPLC analysis time is less than 10 min. The total time of performing analysis per sample is less than 1 h. The important advantage of the proposed method is the capability of determining and reporting α-tocopherol and α-tocopheryl acetate separately. Moreover, the developed method is suitable for fast screening of the synthetic form of vitamin E in dairy products, plant-based milks, infant formulas, and pharmaceutical colostrum. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 19369751
- Volume :
- 10
- Issue :
- 12
- Database :
- Complementary Index
- Journal :
- Food Analytical Methods
- Publication Type :
- Academic Journal
- Accession number :
- 126132939
- Full Text :
- https://doi.org/10.1007/s12161-017-0943-x