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Molecular and immunological characterization of a 64-kDa protein of <em>Actinobacillus actinomycetemcomitans</em>.
- Source :
- Oral Microbiology & Immunology; Jun1995, Vol. 10 Issue 3, p151-159, 9p
- Publication Year :
- 1995
-
Abstract
- The 64-kDa protein to which about half the sera from patients with localized juvenile periodontitis and rapidly progressive periodontitis reacted strongly was purified from <em>Actinobacillus actinomycetemcomitans</em> Y4. Determination of the N-terminal sequence of the protein revealed that it was a GroEL-like protein. The DNA fragment containing the <em>groEL</em> gene of <em>A. actinomycetemcomitans</em> was amplified by polymerase chain reaction, and the <em>groESL</em> operon was cloned by using colony hybridization with the amplified fragment from <em>A. actinomycetemcomitans</em> chromosomal DNA. Sequence analysis revealed that structures of the operon and its products were typical in gram-negative bacteria. Rabbit polyclonal antibodies to the 64-kDa protein cross-reacted with approximately 65-kDa proteins of <em>Haemophilus aphrophilus</em>, <em>Haemophilus influenzae</em>, <em>Haemophilus paraphrophilus</em>, <em>Escherichia coli</em> and <em>Eikenella corrodens</em> but not with any cellular proteins of <em>Porphyromonas gingivalis</em>, <em>Prevotella intermedia</em> and <em>Fusobacteriurn nucleatum</em>. It is possible that antibodies reactive to the 64-kDa protein in periodontitis patients are induced by the cross-reactivity with the hsp60 proteins of other bacteria. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09020055
- Volume :
- 10
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Oral Microbiology & Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 12595546
- Full Text :
- https://doi.org/10.1111/j.1399-302X.1995.tb00136.x