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Nodes of Ranvier in skin biopsies of patients with diabetes mellitus.
- Source :
- Journal of the Peripheral Nervous System; Sep2017, Vol. 22 Issue 3, p182-190, 9p
- Publication Year :
- 2017
-
Abstract
- Paranodal demyelination has been discussed as a potential mechanism of nerve fiber damage in diabetic neuropathy ( DNP). Studies on human tissue are limited, as nerve biopsies are invasive and only rarely performed in patients with confirmed DNP. Skin biopsy has recently been suggested as a tool to analyze paranodal and nodal changes of myelinated fibers. We analyzed the myelinated fibers of skin biopsies of 35 patients with DNP, 17 patients with diabetes mellitus ( DM) without neuropathy, and 30 normal controls. Immunofluorescence of skin sections with antibodies against Caspr, neurofascin, sodium channels, and myelin basic protein was performed to assess paranodal/nodal architecture, segmental demyelination, and myelinated nerve fibers. Staining with antibodies against protein gene product 9.5 was used to quantify unmyelinated nerve fibers. There was an increase of elongated Ranvier nodes and a dispersion of neurofascin at the distal leg in patients with DM with and without neuropathy and at the finger in patients with DNP. An increased dispersion of Caspr was only found in biopsies of the finger in patients with DNP. Skin biopsy may be an appropriate tool to analyze nodes of Ranvier in patients with DM. Structural nodal changes are detectable in DNP and even in diabetic patients without neuropathy. [ABSTRACT FROM AUTHOR]
- Subjects :
- DIAGNOSIS of diabetes
NEURONS
BIOPSY
CELL adhesion molecules
CELL communication
DEMYELINATION
DIABETIC neuropathies
PEOPLE with diabetes
ORGAN donation
FLUORESCENT antibody technique
GLYCOSYLATED hemoglobin
IMMUNOGLOBULINS
NEUROLOGIC examination
RESEARCH funding
SKIN
CONTROL groups
DATA analysis software
DISEASE complications
WOUNDS & injuries
Subjects
Details
- Language :
- English
- ISSN :
- 10859489
- Volume :
- 22
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Journal of the Peripheral Nervous System
- Publication Type :
- Academic Journal
- Accession number :
- 124992969
- Full Text :
- https://doi.org/10.1111/jns.12224