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Selective ATP-Binding Cassette Subfamily C Gene Expression and Proinflammatory Mediators Released by BEAS-2B after PM2.5, Budesonide, and Cotreated Exposures.

Authors :
Encarnación-Medina, Jarline
Rodríguez-Cotto, Rosa I.
Bloom-Oquendo, Joseph
Ortiz-Martínez, Mario G.
Duconge, Jorge
Jiménez-Vélez, Braulio
Source :
Mediators of Inflammation; 8/16/2017, p1-12, 12p
Publication Year :
2017

Abstract

ATP-binding cassette subfamily C (ABCC) genes code for phase III metabolism proteins that translocate xenobiotic (e.g., particulate matter 2.5 (PM<subscript>2.5</subscript>)) and drug metabolites outside the cells. IL-6 secretion is related with the activation of the ABCC transporters. This study assesses ABCC1–4 gene expression changes and proinflammatory cytokine (IL-6, IL-8) release in human bronchial epithelial cells (BEAS-2B) exposed to PM<subscript>2.5</subscript> organic extract, budesonide (BUD, used to control inflammation in asthmatic patients), and a cotreatment (Co-T: PM<subscript>2.5</subscript> and BUD). A real-time PCR assay shows that ABCC1 was upregulated in BEAS-2B exposed after 6 and 7 hr to PM<subscript>2.5</subscript> extract or BUD but downregulated after 6 hr of the Co-T. ABCC3 was downregulated after 6 hr of BUD and upregulated after 6 hr of the Co-T exposures. ABCC4 was upregulated after 5 hr of PM<subscript>2.5</subscript> extract, BUD, and the Co-T exposures. The cytokine assay revealed an increase in IL-6 release by BEAS-2B exposed after 5 hr to PM<subscript>2.5</subscript> extract, BUD, and the Co-T. At 7 hr, the Co-T decreases IL-6 release and IL-8 at 6 hr. In conclusion, the cotreatment showed an opposite effect on exposed BEAS-2B as compared with BUD. The results suggest an interference of the BUD therapeutic potential by PM<subscript>2.5</subscript>. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
09629351
Database :
Complementary Index
Journal :
Mediators of Inflammation
Publication Type :
Academic Journal
Accession number :
124642211
Full Text :
https://doi.org/10.1155/2017/6827194