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Fis R activates σ54-dependent transcription of sulfide-oxidizing genes in C upriavidus pinatubonensis JMP134.

Authors :
Li, Huanjie
Li, Juan
Lü, Chuanjuan
Xia, Yongzhen
Xin, Yufeng
Liu, Honglei
Xun, Luying
Liu, Huaiwei
Source :
Molecular Microbiology; Aug2017, Vol. 105 Issue 3, p373-384, 12p
Publication Year :
2017

Abstract

Some heterotrophic bacteria are able to oxidize sulfide (H<subscript>2</subscript>S, HS<superscript>−</superscript> and S<superscript>2−</superscript>) to sulfite and thiosulfate via polysulfide. The genes coding for the oxidation enzymes in Cupriavidus pinatubonensis JMP134 have recently been identified; however, their regulation is unknown. A regulator gene is adjacent to the operon of the sulfide-oxidizing genes, encoding a σ<superscript>54</superscript>-dependent transcription factor (FisR) with three domains: an R domain, an AAA+ domain and a DNA-binding domain. Here it is reported that the regulator responds to the presence of sulfide and activates the sulfide-oxidizing genes. FisR binds to its cognate operator at −114 to −135 bp of the transcription start of the operon. When polysulfide reacts with the R domain of FisR through the three conserved cysteine residues (C53, C64 and C71), FisR activates the expression of the operon. FisR is highly sensitive to polysulfide, activating σ<superscript>54</superscript>-dependent transcription of sulfide-oxidizing genes for sulfide removal. Further, sequence analysis indicates that FisR-type regulators are relatively common for controlling sulfide-oxidizing genes under sulfide stress in the Proteobacteria. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
0950382X
Volume :
105
Issue :
3
Database :
Complementary Index
Journal :
Molecular Microbiology
Publication Type :
Academic Journal
Accession number :
124256268
Full Text :
https://doi.org/10.1111/mmi.13725