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Mutagenesis and characterization of a Bacillus amyloliquefaciens strain for Cinnamomum camphora seed kernel oil extraction by aqueous enzymatic method.
- Source :
- AMB Express; 7/17/2017, Vol. 7 Issue 1, p1-10, 10p
- Publication Year :
- 2017
-
Abstract
- The purpose of the present study was to increase the proteinase activity of the strain NCU116 by combining ultraviolet irradiation and N-methyl- N′-nitro- N-nitroso guanidine treatment, in order to enhance the efficiency of Cinnamomum camphora seed kernel oil (CCSKO) extraction by aqueous enzymatic method (AEM). The mutated strain, designated as NCU116-1, was screened out by the ratio of hydrolytic zone diameter to colony diameter on skim milk plate. The proteinase activity (9116.1 U/ml) of NCU116-1 was increased by 31.9% compared with the parental strain. The extracellular enzymes produced by NCU116-1 included proteinase, pectase, glucoamylase, cellulase and amylase. The proteinase had the maximum activity at 50 °C. Its optimum temperature and pH value were approximately 45 °C and 8.0 respectively. Mn was an activator of neutral proteinase. The glucoamylase had the maximum activity at 35 °C, and was activated by Cu, Fe and Mn. Its optimum temperatures and pH value were 35 °C and 8.0 respectively. The pectinase had the maximum activity at 40 °C, and was activated by Ca and Mn. Its optimum temperatures and pH value were 35-40 °C and 6.0 respectively. The optimum conditions of CCSKO extraction by AEM were also investigated. The results suggested that the best amount of enzyme solution and enzymolysis time were 20% (v/v) and 4 h, respectively. The oil extraction rate was 95.2% under these conditions. Thus, a suitable mutated strain was selected for CCSKO extraction by AEM and the optimum extraction conditions were determined. [ABSTRACT FROM AUTHOR]
- Subjects :
- BACILLUS amyloliquefaciens
MUTAGENESIS
CINNAMOMUM
PLANT products
EDIBLE fats & oils
Subjects
Details
- Language :
- English
- ISSN :
- 21910855
- Volume :
- 7
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- AMB Express
- Publication Type :
- Academic Journal
- Accession number :
- 124176440
- Full Text :
- https://doi.org/10.1186/s13568-017-0451-9