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Maternal microchimerism is prevalent in cord blood in memory T cells and other cell subsets, and persists post-transplant.
- Source :
- OncoImmunology; 2017, Vol. 6 Issue 5, pN.PAG-N.PAG, 1p
- Publication Year :
- 2017
-
Abstract
- Among reported advantages of umbilical cord blood (CB) in transplantation is lower leukemia relapse probability. Underlying cellular mechanisms of graft-vs.-leukemia (GVL) are thought to include a prominent role for T cells. Cells of the CB's mother, maternal microchimerism (MMc), were recently strongly, but indirectly, implicated in this GVL benefit. We assayed MMc directly and hypothesized benefit accrues from CB maternal T cells. MMc was quantified in 51 CBs and, within memory T, naïve T, B, NK cells, and monocytes in 27 CBs. Polymorphism-specific quantitative-PCR assays targeted maternal genotypes non-shared with CBs. Overall MMc was common and often at substantial levels. It was present in 52.9% of CB and in 33.3–55.6% of tested subsets. Remarkably, MMc quantities were greater in memory T cells than other subsets (p< 0.001). Expressed as genome equivalents (gEq) per 105total gEq tested (gEq/105), memory T cell MMc averaged 850.2 gEq/105, while other subset mean quantities were 13.8–30.1 gEq/105. After adjustment for proportionality in CB, MMc remained 6–17 times greater in memory T, and 3–9 times greater in naïve T, vs. non-T-cell subsets. Further, CB-origin MMc was detectedin vivoin a patient up to 6 mo post-transplantation, including among T cells. Overall, results revealed levels and phenotypes of CB MMc with potential relevance to CB transplantation and, more broadly, to offspring health. [ABSTRACT FROM PUBLISHER]
- Subjects :
- CORD blood transplantation
CHIMERISM
LEUKEMIA
CANCER relapse
T cells
Subjects
Details
- Language :
- English
- ISSN :
- 21624011
- Volume :
- 6
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- OncoImmunology
- Publication Type :
- Academic Journal
- Accession number :
- 123479389
- Full Text :
- https://doi.org/10.1080/2162402X.2017.1311436