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Sex pheromone plasmid pad1-encoded aggregation substance of Enterococcus faecalis is positively regulated in trans by traE1.
- Source :
- European Journal of Biochemistry; 5/15/93, Vol. 214 Issue 1, p333-338, 6p
- Publication Year :
- 1993
-
Abstract
- Sex-pheromone-plasmid-bearing strains of Enterococcus faecalis react with sex pheromone to induce the expression of an adhesin, the so-called aggregation substance, on their cell surface. Here we show that, by complementation studies, for sex-pheromone plasmid pAD1, expression of the structural gene asa1, coding for an aggregation substance, is mediated by a diffusible factor encoded on pAD1. We were able to demonstrate that a small open reading frame, traE1,is sufficient for transcription of the operon containing asa1. A model for expression of asa1 under the influence of the positive regulator is presented, which is supported by our observation that regulation involves an all-or-nothing induction phenomenon, leading to cells either fully expressing asa1 or not at all. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00142956
- Volume :
- 214
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- European Journal of Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 12288012
- Full Text :
- https://doi.org/10.1111/j.1432-1033.1993.tb17928.x