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PMScl-75 is the main autoantigen in patients with the polymyositis/scleroderma overlap syndrome.
- Source :
- Arthritis & Rheumatism; Feb2004, Vol. 50 Issue 2, p565-569, 5p
- Publication Year :
- 2004
-
Abstract
- To compare the autoantigenicity of the recently described N-terminally elongated PMScl-75 protein with that of PMScl-100 and the originally defined PMScl-75 polypeptide, and to determine its value for analyzing sera from patients with the polymyositis (PM)/scleroderma overlap syndrome. Serum samples obtained from patients with the PM/scleroderma overlap syndrome and from patients with several other diseases were analyzed for the presence of autoantibodies reactive with recombinant PMScl-100 and PMScl-75 (both the original and the longer form) proteins, in an enzyme-linked immunosorbent assay (ELISA). Autoantibodies recognizing the longer PMScl-75 protein isoform were detected in 28% of the patients with PM/scleroderma. This percentage is slightly higher than that for PMScl-100 (25%) and is significantly higher than that for the previously defined PMScl-75 protein (11%). In addition, we identified a significant number of patients who had antiPMScl-75 but not antiPMScl-100 antibodies. This finding contrasts with what has been previously reported for the shorter version of the PMScl-75 protein. Our data indicate that use of the long PMScl-75 isoform in addition to PMScl-100 in ELISAs significantly increases the number of patients in whom antiPM-Scl autoantibodies can be detected. [ABSTRACT FROM AUTHOR]
- Subjects :
- PROTEINS
SCLERODERMA (Disease)
AUTOANTIBODIES
POLYMYOSITIS
MYOSITIS
Subjects
Details
- Language :
- English
- ISSN :
- 00043591
- Volume :
- 50
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Arthritis & Rheumatism
- Publication Type :
- Academic Journal
- Accession number :
- 12266808
- Full Text :
- https://doi.org/10.1002/art.20056