Start Over

Structural Characterization of RNA Recognition Motif-2 Domain of SART3.

Authors :: Bang, Kyeong-Mi
Cho, Na Youn
Kim, Won-Je
Kim, Ae-Ryung
Song, Hyun Kyu
Kim, Eunice EunKyeong
Kim, Nak-Kyoon
Source :: Bulletin of the Korean Chemical Society; Apr2017, Vol. 38 Issue 4, p444-447, 5p
Publication Year :: 2017
Abstract: Squamous cell carcinoma antigen recognized by T-cells 3 ( SART3) is an essential recycling factor in pre- mRNA splicing, which is required for association of U4/ U6 small nuclear ribonucleoprotein ( snRNP). SART3 contains two RNA recognition motifs ( RRMs), and they are responsible for the tertiary interaction with U6 small nuclear RNA. Despite the importance of structural studies for understanding complicate U4/ U6 snRNP recycling mechanism, only a few of them have been performed for SART3. Here, the structure of SART3 RRM2 was characterized by heteronuclear multi-dimensional nuclear magnetic resonance experiments. Nearly complete <superscript>1</superscript>H, <superscript>15</superscript>N, and <superscript>13</superscript>C chemical shifts of the backbone residues of RRM2 were assigned. In addition, the secondary structure of RRM2 were predicted by the chemical shift index and TALOS+ analyses, and the results showed that RRM2 forms a 'β<subscript>1</subscript>-α<subscript>1</subscript>-β<subscript>2</subscript>-β<subscript>3</subscript>-α<subscript>2</subscript>-β<subscript>4</subscript>-β<subscript>5</subscript>' structure, where β<subscript>4</subscript> is not common in the canonical RRM domain structures. Our results will provide structural basis for investigation of SART3-mediated U4/ U6 snRNP complex formation. [ABSTRACT FROM AUTHOR]