A Mutant Elongation Factor Tu which Does Not Immobilize the Ribosome upon Binding of Kirromycin.

Focuses on the mutant elongation factor Tu which does not immobilize the ribosome upon binding of kirromycin. Search for the primary site of inhibition in the elongation cycle; Formation by the elongation factor TuB[sub o] of a complex with aminoacyl-tRNA and guanosine triphosphate; Ability of the complex to bind to ribosomes programmed with poly; Recessive nature of the tuf B mutation in cells producing kirromycin-resistant elongation factor TuA and TuB[sub o]; Polypeptide synthesis; Ability to bind the antibiotic.