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Evaluation of the p-AKT, p-JNK and FoxO3a function in oral epithelial dysplasia.
- Source :
- Oral Diseases; Apr2017, Vol. 23 Issue 3, p367-378, 12p, 3 Diagrams, 4 Charts
- Publication Year :
- 2017
-
Abstract
- Objectives To evaluate the expression of p-AKT, p-JNK, FoxO3a, and Ki-67 in samples of oral squamous cell carcinoma (OSCC) and oral epithelial dysplasias (OEDs) to understand their possible involvement in the malignant transformation process of oral lesions. Materials and Methods Tissue samples of 20 cases of OSCCs, 20 OEDs, and normal oral mucosa were subjected to immunohistochemistry reactions for anti-p-AKT, anti-p-JNK, anti-FoxO3a, and anti-Ki-67 antibodies. It was analyzed using quantitative (number of immunostained cells) and qualitative (immunostaining intensity) parameters in different cell immunostaining sublocations. Results Nuclear p-AKT was observed significantly greater immunostaining in OSCC (21.2 ± 19.0) than in dysplasias (7.9 ± 8.1) and controls (1.8 ± 4.7) ( P = 0.002). Immunostaining of strong nuclear p-JNK was greater in controls (48.3 ± 13.7) than in OEDs (11.0 ± 10.3) and OSCCs (1.1 ± 1.3) ( P < 0.001). Strong nuclear immunostaining of FoxO3a proved to be absent in OSCCs (0.0 ± 0.1) with little staining on dysplasias (3.2 ± 5.4) and increased expression in controls (13.5 ± 4.8) ( P < 0.001). Immunostaining of strong nuclear Ki-67 was grater in OSCCs (48.1 ± 49.6) than in OED (11.8 ± 10.6) and controls (1.9 ± 2.0) ( P < 0.001). Conclusions Malignant process of OEDs in this research may involve the same mechanisms of established malignant lesions. [ABSTRACT FROM AUTHOR]
- Subjects :
- ANALYSIS of variance
CONFIDENCE intervals
GENE expression
IMMUNOGLOBULINS
IMMUNOHISTOCHEMISTRY
MOUTH tumors
SCIENTIFIC observation
ORAL mucosa
PRECANCEROUS conditions
PROBABILITY theory
PROTEINS
RESEARCH funding
SQUAMOUS cell carcinoma
STAINS & staining (Microscopy)
STATISTICS
T-test (Statistics)
DATA analysis
CROSS-sectional method
DATA analysis software
DESCRIPTIVE statistics
NEOPLASTIC cell transformation
ODDS ratio
Subjects
Details
- Language :
- English
- ISSN :
- 1354523X
- Volume :
- 23
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Oral Diseases
- Publication Type :
- Academic Journal
- Accession number :
- 121625107
- Full Text :
- https://doi.org/10.1111/odi.12623